Molecular biology

Explore the exciting world of molecular biology and learn about the latest discoveries and breakthroughs. Uncover the secrets of life at the molecular level and see how it impacts our understanding of genetics, diseases, and more.
8.7: DNA Analysis- Blotting and Hybridization - Biology LibreTexts Biology Classroom, Anatomy And Physiology, Life Science, Dna Jewelry, Organic Chemistry, Technology Gadgets, Higher Education, Science Nature, Learning

Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.