The Countess of Pembroke's husband died in 1600. Her husband's will required that she did not remarry. Thereafter, her time was spent managing Wilton House and the other Pembroke estates, on behalf of her son, William Herbert, 3rd Earl of Pembroke, who followed in his mother's footsteps as a literary patron. After James I visited her at Wilton in 1603 and was entertained by Shakespeare's company, The King's Men, Mary moved out of Wilton as Dowager Countess and rented homes in London.